Veneno de abejas, comunicación (2024)

  miércoles 27 de noviembre de 2024

Volume 07, Issue 06 (November-December 2024), PP 44-48 www.ijmsdr.org ISSN: 2581-902X 44 

 International Journal of Medical Science and Dental Research 

Short Comunication: Proposal Standardize Method for Characterization and Quantification of Bee Venom in Honeybee Apis mellifera by High Performance Liquid Chromatography (HPLC)

 Prada-Ramírez Harold Alexis1 , and Peña-Romero Romel1 1 (Chemistry Department / Coaspharma S.A.S, Colombia) 

Despite the well-known chemical characterization of bee venom that enable to identify the three major peptides such as apamin, phospholipase A2, and melittin through HPLC test its chromatographic condition use to be diverse (mobile and stationary phases). The main purpose of this manuscript is to summarize the suitable HPLC condition for characterization and quantification of bee venom in Colombia honeybee (Apis mellifera) based on a literature review. Bee venom is often collected using the electrostimulation method because this cutting-edge technology prevents bee deaths as the bee venom is efficiently extracted. The bee venom samples normally are stored under suitable conditions to prevent degradation such as autohydrolysis (frozen conditions). According to scientific evidence gathered from published article High-Performance Liquid Chromatographic method is usually used to assay the major venom compounds as melittin, apamin, and phospholipase A2. However, its running conditions seem to be diverse. In the present manuscript we aim to review the optimal HPLC conditions to separate the mains bee venom compounds. Therefore, according to literature review the best Chromatographic separation was performed using the following mobile phases: A - 0.1% trifluoroacetic acid (TFA) in water, B - 0.1% TFA in acetonitrile: water (80:20). The assays of the separated venom compounds were made using a UV detector at 220 nm wavelength. The stationary phase is carried out using a chromatographic column with C18 packing materials. The proposal HPLC running condition allows an adequate separation of the major HPLC peaks. As it has been previously observed in Apis Mellifera syriaca and Apis mellifera meda the bee venom samples showed up the expected chromatographic profile that allows to identify the three major protein fraction compounds: melittin, phospholipase A2 and apamine. Therefore, those chromatographic conditions could be widely used to perform routine bee venom assessments in the pharmaceutical industry. Efficacy of apitoxin extraction through the electrostimulation method has been shown to work well since it allows to identify the three major peptides of the bee venom as follows apamin, phospholipase A2, and melittin using HPLC.

 Keywords – Apis mellifera, HPLC, bee venom, Apitoxin, melittin, apamin, phospholipase A2. Volume 07, Issue 06 (November-December 2024), PP 44-48 www.ijmsdr.org ISSN: 2581-902X 

https://ijmsdr.org/published%20paper/1i1i37/Short-Comunication-Proposal-Standardize-Method-for-Characterization-and-Quantification-of-Bee-Venom-in-Honeybee-Apis-mellifera-by-High-Performance-Liquid-Chromatography-HPLC.pdf